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Isolation of plasma membranes from Drosophila embryos by Quiao-Yun Jiang; A. Gnagey; B. Tandler; M. Jacobs-Lorena is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Isolation of plasma membranes from Drosophila embryos about?

Cell surface components probably play an important role in early embryonic development. However, hardly any information is available on the structure or regulation of expression of the corresponding genes. As a first step in approaching this issue, we devised a procedure to obtain enriched plasma membranes from embryonic Drosophila ceils. Membranes are fractionated according to two independent physical parameters: size, using velocity gradient centrifugation and density, using isopicnic gradient centrifugation. The final membrane fraction is enriched by 6 to 8 fold with respect to the plasma membrane enzyme marker Na+/K+ ATPase and substantially depleted of the mitochondrial enzyme marker cytochrome C oxidase. Two-dimensional polyacrylamide gel electrophoresis of the purified membranes reveals enrichment for specific proteins and electron microscopy reveals membrane vesicles in abundance. The enriched fraction should be suitable for the preparation of antibody probes that recognize cell surface components.

Who reads Isolation of plasma membranes from Drosophila embryos?

Author

Quiao-Yun Jiang; A. Gnagey; B. Tandler; M. Jacobs-Lorena

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0301-4851)

Published

1985

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)