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Genetic dissection of the \ \ miR-17∼92\ \ cluster of microRNAs in Myc-induced B-cell lymphomas by Ping Mu; Yoon-Chi Han; Doron Betel; Evelyn Yao; Massimo Squatrito; Paul Ogrodowski; Elisa de Stanchina; Aleco D'Andrea; Chris Sander; Andrea Ventura is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genetic dissection of the \ \ miR-17∼92\ \ cluster of microRNAs in Myc-induced B-cell lymphomas about?

The __miR-17∼92__ cluster is frequently amplified or overexpressed in human cancers and has emerged as the prototypical oncogenic polycistron microRNA (miRNA). __miR-17∼92__ is a direct transcriptional target of c-Myc, and experiments in a mouse model of B-cell lymphomas have shown cooperation between these two oncogenes. However, both the molecular mechanism underlying this cooperation and the individual miRNAs that are responsible for it are unknown. By using a conditional knockout allele of __miR-17∼92__, we show here that sustained expression of endogenous __miR-17∼92__ is required to suppress apoptosis in Myc-driven B-cell lymphomas. Furthermore, we show that among the six miRNAs that are encoded by __miR-17∼92__, __miR-19a__ and __miR-19b__ are absolutely required and largely sufficient to recapitulate the oncogenic properties of the entire cluster. Finally, by combining computational target prediction, gene expression profiling, and an in vitro screening strategy, we identify a subset of __miR-19__ targets that mediate its prosurvival activity.

Who reads Genetic dissection of the \ \ miR-17∼92\ \ cluster of microRNAs in Myc-induced B-cell lymphomas?

Author

Ping Mu; Yoon-Chi Han; Doron Betel; Evelyn Yao; Massimo Squatrito; Paul Ogrodowski; Elisa de Stanchina; Aleco D'Andrea; Chris Sander; Andrea Ventura

Publisher

Cold Spring Harbor Laboratory

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)