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Steps for Creating Stable Cell Lines by Yamuna Joseph is a document available to read on EtoBox.
What is Steps for Creating Stable Cell Lines about?
This document provides a procedure for establishing stable cell lines that constitutively express GFP-tagged proteins. Key steps include: 1. Cloning the protein of interest into a GFP vector and confirming expression and localization in transiently transfected cells. 2. Transfecting cells with the GFP construct and selecting for stable integrants using G418 antibiotic selection over 1-2 weeks. 3. Isolating individual colonies, expanding them, and screening for clones with uniform GFP expression in >95
- Author
- Yamuna Joseph
- Language
- EN