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In vitro characterization of a slow release system of polylactic acid and rhBMP2 by H. Schliephake; H. A. Weich; J. Schulz; R. Gruber is a Engineering article available to read on EtoBox.

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## Abstract The aim of the present report was to test a system for controlled release of recombinant human bone morphogenic protein (rhBMP2) incorporated into polylactic acid (PLA) implants. Incorporation of rhBMP2 into the polymer was accomplished by mixing rhBMP2 solution with granular powder of amorphous poly‐DL‐lactic acid, subsequent lyophilization, and high pressure CO~2~ treatment at 100 bar. Porous cylindrical implants of 8 mm diameter and 3 mm thickness were fabricated with 100, 200, 400, and 800 μg BMP2/g polymer and submitted to __in vitro__ testing. Polymer degradation was assessed during immersion of PLA implants into PBS for 176 days by measuring the inherent viscosity at days 0, 99, and 131. BMP2 release was evaluated by immersion of both the lyophilized powder and the implants into cell culture medium for up to 27 days. BMP2 release was assessed using a custom made ELISA. The biological activity of the released growth factor was determined by measuring the induction of alkaline phosphatase (AP) in C2C12 cells. There was a significant retardation in the release of BMP2 from the implants compared to the granular powder. Detectable amounts of BMP2 were found for all co

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Author

H. Schliephake; H. A. Weich; J. Schulz; R. Gruber

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)