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The MNS Blood Group Antigens, Vr (MNS12) and Mt^a^ (MNS14), Each Arise from an Amino Acid Substitution on Glycophorin A by Jill R. Storry; Gail Coghlan; Joyce Poole; Dolores Figueroa; Marion E. Reid is a Medicine article available to read on EtoBox.

What is The MNS Blood Group Antigens, Vr (MNS12) and Mt^a^ (MNS14), Each Arise from an Amino Acid Substitution on Glycophorin A about?

## Abstract **__Background and Objectives:__** The antigens, Vr (MNS12) and Mt^a^ (MNS14), are low‐incidence antigens of the MNS blood group system. The Vr antigen has been found only on the red blood cells (RBCs) of persons of Dutch ancestry whereas the Mt^a^ antigen has been found on the RBCs of persons from a wide geographic distribution. The objective of this study was to determine the molecular basis of Vr and Mt^a^. **__Materials and Methods:__** Following RT‐PCR amplification of total RNA isolated from one Vr+ person (G488) and one Mt(a+) person (GH), the genes encoding glycophorin A __(GYPA)__ and glycophorin B __(GYPB)__ were cloned and sequenced. To confirm the point mutation observed in the cDNA from G488 (Vr+), __GYPA__ exon 3 was cloned and sequenced from the genomic DNA of G488 and a second unrelated Vr+ person (MU). A restriction fragment length polymorphism (RFLP) assay was used to analyze genomic DNA from 11 Mt(a+) persons (10 unrelated) following PCR amplification of __GYPA__ exon 3. **__Results:__** The coding sequence of __GYPB__ was normal in both G488 (Vr+) and GH (Mt(a+)). Sequencing data from __GYPA__ clones derived from G488 showed ot full length __GYPA__ s

Who reads The MNS Blood Group Antigens, Vr (MNS12) and Mt^a^ (MNS14), Each Arise from an Amino Acid Substitution on Glycophorin A?

Author

Jill R. Storry; Gail Coghlan; Joyce Poole; Dolores Figueroa; Marion E. Reid

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0042-9007)

Published

2000

Language

EN

Field

Medicine (Health Sciences)