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Optimization and Validation of a Method for the Determination of Caffeine, 8-Chlorotheophylline and Diphenhydramine by Isocratic High-performance Liquid Chromatography by Coral Barbas; Antonia Garcı́a; Luis Saavedra; Mario Castro is a Chemistry article available to read on EtoBox.

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The optimization of a HPLC method for caffeine, 8-chlorotheophylline and diphenhydramine separation with UV detection at 229 nm is described. The conditions studied included: stationary phase, compositions of mobile phases with pH modulators. Optimal conditions were: SymmetryShield RP8 column and acetonitrile-(0.01 M H PO -triethylamine, pH 2.8) 3 4 (22:78, v / v). Validation was performed using standards and a pharmaceutical preparation containing the compounds described above. Results from both standards and samples show suitable validation parameters. The pharmaceutical grade substances were tested by factors that could influence the chemical stability. These reaction mixtures were analyzed to evaluate the capability of the method to separate degradation products. Degradation products did not interfere with the determination of the substances tested by the assay.

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Author

Coral Barbas; Antonia Garcı́a; Luis Saavedra; Mario Castro

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)