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A Proteomic Study of the Arabidopsis Nuclear Matrix by Tomasz T. Calikowski; Tea Meulia; Iris Meier is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Proteomic Study of the Arabidopsis Nuclear Matrix about?

## Abstract The eukaryotic nucleus has been proposed to be organized by two interdependent nucleoprotein structures, the DNA‐based chromatin and the RNA‐dependent nuclear matrix. The functional composition and molecular organization of the second component have not yet been resolved. Here, we describe the isolation of the nuclear matrix from the model plant Arabidopsis, its initial characterization by confocal and electron microscopy, and the identification of 36 proteins by mass spectrometry. Electron microscopy of resinless samples confirmed a structure very similar to that described for the animal nuclear matrix. Two‐dimensional gel electrophoresis resolved approximately 300 protein spots. Proteins were identified in batches by ESI tandem mass spectrometry after resolution by 1D SDS–PAGE. Among the identified proteins were a number of demonstrated or predicted Arabidopsis homologs of nucleolar proteins such as IMP4, Nop56, Nop58, fibrillarins, nucleolin, as well as ribosomal components and a putative histone deacetylase. Others included homologs of eEF‐1, HSP/HSC70, and DnaJ, which have also been identified in the nucleolus or nuclear matrix of human cells, as well as a number o

Who reads A Proteomic Study of the Arabidopsis Nuclear Matrix?

Author

Tomasz T. Calikowski; Tea Meulia; Iris Meier

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)