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Dopaminergic differentiation of the Nurr1‐expressing immortalized mesencephalic cell line CSM14.1 in vitro by Stefan Jean‐Pierre Haas; Andreas Wree is a Earth and Planetary Sciences article available to read on EtoBox.

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## Abstract The use of neural stem cells as grafts is a potential treatment for Parkinson's disease, but the potential of stem cells to differentiate into dopaminergic neurones requires investigation. The present study examined the __in vitro__ differentiation of the temperature‐sensitive immortalized mesencephalic progenitor cell line CSM14.1 under defined conditions. Cells were derived from the mesencephalic region of a 14‐day‐old rat embryo, retrovirally immortalized with the Large T antigen and cultured at 33 °C in DMEM containing 10% fetal calf serum (FCS). For differentiation, the temperature was elevated at 39 °C and FCS was reduced (1%). Using histology, immunocytochemical detection of the stem cell marker Nestin and the neuronal marker MAP5 and, in addition, Western blotting to determine the presence of neurone‐specific enolase and the neurone nuclei antigen we demonstrated a differentiation of these cells into neuronal cells accompanied by a decrease in Nestin production. In Western blots, we detected the orphan nuclear receptor Nurr1 in these cells. This was followed by a time‐dependent up‐regulation of the enzymes tyrosine hydroxylase and aldehyde dehydrogenase 2 charac

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Author

Stefan Jean‐Pierre Haas; Andreas Wree

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0021-8782)

Published

2002

Language

EN

Field

Earth and Planetary Sciences (Physical Sciences)