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This study identifies and characterizes 39 interleukin (IL) genes in grass carp (Ctenopharyngodon idella), revealing their evolutionary relationships and potential roles in immune responses to GCRV and Aeromonas hydrophila challenges. The IL genes were classified into six families, with expression profiles indicating significant regulation during immune challenges. This research provides a foundational understanding of ILs in grass carp, paving the way for further functional investigations.

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