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DNA requirements for assembly and stability of HIV‐1 intasomes by Min Li; Vassili Ivanov; Michiyo Mizuuchi; Kiyoshi Mizuuchi; Robert Craigie is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA requirements for assembly and stability of HIV‐1 intasomes about?

## Abstract Integration of viral DNA into the host genome is an essential step in retroviral replication that is mediated by a stable nucleoprotein complex comprising a tetramer of integrase bridging the two ends of the viral DNA in a stable synaptic complex (SSC) or intasome. Assembly of HIV‐1 intasomes requires several hundred base pairs of nonspecific internal DNA in addition to the terminal viral DNA sequence that is protected in footprinting experiments. We find that only one of the viral DNA ends in the intasome requires long‐nonspecific internal DNA for intasome assembly. Although intasomes are unstable in solution when the nonspecific internal DNA is cut off after assembly, they are stable in agarose gels. These complexes are indistinguishable from SSCs with nonspecific internal DNA in Förster resonance energy transfer (FRET) experiments suggesting the interactions with the viral DNA and integrase tetramer are the same regardless of the presence of nonspecific internal DNA. We discuss models of how the internal DNA contributes to intasome assembly and stability. FRET is exquisitely sensitive to the distance between the fluorophores and given certain assumptions can be trans

Who reads DNA requirements for assembly and stability of HIV‐1 intasomes?

Author

Min Li; Vassili Ivanov; Michiyo Mizuuchi; Kiyoshi Mizuuchi; Robert Craigie

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

2012

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)