About this Medicine article

Differential Screening of a Human Pancreatic Adenocarcinoma λGt11 Expression Library Has Identified Increased Transcription of Elongation Factor Ef‐1α in Tumour Cells by Anne G. Grant; Rachel M. Flomen; Mark L. V. Tizard; David A. W. Grant is a Medicine article available to read on EtoBox.

## Abstract A human pancreatic adenocarcinoma λgt11 expression library was differentially screened with mRNA derived from normal and cancerous pancreatic tissues. Five clones preferentially hybridized with pancreatic adenocarcinoma mRNA. cDNA inserts from 4 of these clones were amplified by PCR, labelled with α^32^P and used in Northern blot analysis against mRNA prepared from a variety of tumour and normal tissues. λGER‐4 identified a pancreas‐associated mRNA (> 10 kb) with no homology with known sequences at either the nucleic or amino‐acid level. λGER‐2 identified a 1.7‐kb mRNA transcript that was over‐expressed in mRNA prepared from pancreas, colon, breast, lung and gastric tumours relative to normal tissues. Sequence analysis and restriction‐enzyme mapping showed that this clone was completely homologous with the active form of human elongation factor EF‐1α. This high level of EF‐1α‐mRNA expression in tumour tissues lends support to the increasing evidence that EF‐1α is an important regulator of the cell cycle.

Author

Anne G. Grant; Rachel M. Flomen; Mark L. V. Tizard; David A. W. Grant

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1992

Language

EN

Field

Medicine (Health Sciences)