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Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts by Nelly Bosselut; Chantal Housset; Paulo Marcelo; Colette Rey; Thorsten Burmester; Jöelle Vinh; Michel Vaubourdolle; Axelle Cadoret; Bruno Baudin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts about?
## Abstract In chronic liver diseases, the accumulation of extracellular matrix leading to fibrosis is caused by myofibroblasts, the origins of which are debatable. We performed a comparative proteomic study to identify markers and gain insight into distinct functions of myofibroblasts derived either from hepatic stellate cells (HSCs) or from portal mesenchymal cells. After isolation from normal liver and culture in similar conditions, myofibroblastic HSCs (MF‐HSCs) presented enlarged cytoplasms whereas portal myofibroblasts (PMFs) were more proliferative, and formed more stress fibers. The two cell types were subjected to comparative analyses by 2‐D MS/MS. Six proteins were overexpressed in PMFs, with myofibroblast‐related typical functions. Among them, cofilin‐1 showed the greatest difference in expression and a lower p__I__ than expected. Immunoblot demonstrated higher levels of phosphorylation, a modification of the protein implicated in stress fiber formation. Eleven proteins, mostly involved in stress response, were overexpressed in MF‐HSCs. Cytoglobin had the highest level of overexpression, as confirmed by reverse transcription quantitative real‐time PCR, immunoblot and imm
Who reads Distinct proteomic features of two fibrogenic liver cell populations: Hepatic stellate cells and portal myofibroblasts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nelly Bosselut; Chantal Housset; Paulo Marcelo; Colette Rey; Thorsten Burmester; Jöelle Vinh; Michel Vaubourdolle; Axelle Cadoret; Bruno Baudin
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)