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The study demonstrates the application of polymerase chain reaction (PCR) for diagnosing fowl poxvirus (FPV) infections by amplifying a specific 578-bp fragment of the FPV genome. The PCR method showed high sensitivity and specificity, successfully detecting FPV in infected chicken tissues while yielding no results from uninfected controls or unrelated avian pathogens. This technique offers a rapid and cost-effective alternative to traditional diagnostic methods for FPV, which are often lengthy and expensiv

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