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Production of Monoclonal Antibodies to Islet Cell Surface Antigens Using Hybridization of Spleen Lymphocytes from Non-obese Diabetic Mice by K. Yokono; K. Shii; J. Hari; S. Yaso; Y. Imamura; K. Ejiri; K. Ishihara; S. Fujii; T. Kazumi; H. Taniguchi; S. Baba is a Medicine article available to read on EtoBox.
What is Production of Monoclonal Antibodies to Islet Cell Surface Antigens Using Hybridization of Spleen Lymphocytes from Non-obese Diabetic Mice about?
Non-obese diabetic mice display a syndrome with dramatic clinical and pathological features similar to those of Type 1 (insulin-dependent) diabetes in man. Circulating autoantibodies to the surface of islet cells were demonstrated in some of these mice by a protein A radioligand assay. To produce monoclonal antibodies to islet cell surface antigens, therefore, we took the spleens of non-obese diabetic mice, transferred the spleen cells into non-immunized recipient mice, which were made immunologically incompetent by a large dose of X-irradiation, and then fused their lymphocytes with FO mouse myeloma cells. After screening the resultant hybrids, one stable hybridoma (3A4) that produced a monoclonal antibody (IgG1) specifically bound to the surface of islet cells was obtained. The purified monoclonal antibody was bound to the surface of transplantable Syrian golden hamster insulinoma cells sevenfold more than control antibody. Adsorption of the antibody on mouse spleen lymphocytes or thymocytes resulted in only a slight decrease in 125I-protein A binding to insulinoma cells. This antibody also reacted with the surface of mouse and rat islet cells, but not with that of rat spleen cel
Who reads Production of Monoclonal Antibodies to Islet Cell Surface Antigens Using Hybridization of Spleen Lymphocytes from Non-obese Diabetic Mice?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- K. Yokono; K. Shii; J. Hari; S. Yaso; Y. Imamura; K. Ejiri; K. Ishihara; S. Fujii; T. Kazumi; H. Taniguchi; S. Baba
- Publisher
- Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0012-186X)
- Published
- 1984
- Language
- EN
- Field
- Medicine (Health Sciences)