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Normobaric hyperoxia protects the blood brain barrier through inhibiting Nox2 containing NADPH oxidase in ischemic stroke by Wenlan Liu; Qingquan Chen; Jie Liu; Ke Jian Liu is a Medicine article available to read on EtoBox.

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Normobaric hyperoxia (NBO) has been shown to be neuro- and vaso-protective during ischemic stroke. However, the underlying mechanisms remain to be fully elucidated. Activation of NADPH oxidase critically contributes to ischemic brain damage via increase in ROS production. We herein tested the hypothesis that NBO protects the blood-brain barrier (BBB) via inhibiting gp91^phox^ (or called Nox2) containing NADPH oxidase in a mouse model of middle cerebral artery occlusion (MCAO). Wild-type C57/BL6 mice and gp91^phox^knockout mice were given NBO (95% O~2~) or normoxia (21% O~2~) during 90-min MCAO, followed by 22.5 hrs of reperfusion. BBB damage was quantified by measuring Evans blue extravasation. The protein levels of matrix metalloproteinase-9 (MMP-9), tight junction protein occludin and gp91^phox^ were assessed with western blot. Gel zymography was used to assess the gelatinolytic activity of MMP-9. In the wild type mice, cerebral ischemia and reperfusion led to remarkable Evans blue extravasation, significantly increased gp91^phox^ and MMP-9 levels and decreased occludin levels in the ischemic brain tissue. In gp91^phox^ knockout mice, the changes in Evans blue extravasation, MMP-

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Author

Wenlan Liu; Qingquan Chen; Jie Liu; Ke Jian Liu

Publisher

BioMed Central; Medknow Publications; London: BioMed Central; Mumbai: Wolters Kluwer - Medknow; Springer Science and Business Media LLC; Medknow (ISSN 2045-9912)

Published

2011

Language

EN

Field

Medicine (Health Sciences)