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Complete Genome Sequence of an Aerobic Hyper-thermophilic Crenarchaeon, Aeropyrum pernix K1 by Y. Kawarabayasi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix Kl, which optimally grows at 95°C, has been determined by the whole genome shotgun method with some modifications. The entire length of the genome was 1,669,695 bp. The authenticity of the entire sequence was supported by restriction analysis of long PCR products, which were directly amplified from the genomic DNA. As the potential protein-coding regions, a total of 2,694 open reading frames (ORFs) were assigned. By similarity search against public databases, 633 (23.5%) of the ORFs were related to genes with putative function and 523 (19.4%) to the sequences registered but with unknown function. All the genes in the TCA cycle except for that of alpha-ketoglutarate dehydrogenase were included, and instead of the alpha-ketoglutarate dehydrogenase gene, the genes coding for the two subunits of 2-oxoacid:ferredoxin oxidoreductase were identified. The remaining 1,538 ORFs (57.1%) did not show any significant similarity to the sequences in the databases. Sequence comparison among the assigned ORFs suggested that a considerable member of ORFs were generated by sequence duplication. The RNA

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Author

Y. Kawarabayasi

Publisher

Oxford University Press (OUP)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)