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Melatonin induces mitochondrial apoptosis in osteoblasts by regulating the STIM1/cytosolic calcium elevation/ERK pathway by Shui Qiu; Zheng Bo Tao; Lin Tao; Yue Zhu is a Neuroscience article available to read on EtoBox.

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## Aims: Idiopathic scoliosis is a common deformity of the spine that has an especially high incidence rate in adolescents. some studies have demonstrated a close relationship between idiopathic scoliosis and melatonin deficiency. our team's previous research showed that melatonin can inhibit the proliferation of osteoblasts, but the mechanism remains unclear. this study aimed to determine the mechanism by which melatonin inhibits the proliferation of osteoblasts. ## Main methods: Cell viability experiment, dna fragment detection and alkaline phosphatase (alp) activity assays were performed to determine the effects of melatonin on the proliferation, apoptosis and differentiation of osteoblasts. we used immunofluorescence to detect the expression of stim1 in melatonin-treated osteoblasts. stim1 interference was achieved using a specific sirna, and a trpc inhibitor was used to block the influx of ca. the mrna expression was determined by rt-qpcr, and protein levels were measured by western blot. ## Key findings: In this study, we found that melatonin inhibited the proliferation, differentiation and apoptosis of osteoblasts in a concentration-dependent manner. additional studies showe

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Author

Shui Qiu; Zheng Bo Tao; Lin Tao; Yue Zhu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0024-3205)

Published

2020

Language

EN

Field

Neuroscience (Life Sciences)