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A Selective HIV-Protease Assay Based on a Chromogenic Amino Acid by Fabrizio Badalassi; Hong Khan Nguyen; Paolo Crotti; Jean-Louis Reymond is a Chemistry article available to read on EtoBox.

What is A Selective HIV-Protease Assay Based on a Chromogenic Amino Acid about?

Dedicated to Professor Dieter Seebach on the occasion of his 65th birthday (2S,3S)-2-Amino-3-hydroxy-5-(4-nitrophenoxy)pentanoic acid (5) was prepared stereoselectively as the N-Fmoc, O-(tert-butyl)-protected derivative 5a in eleven steps from ethyl (E)-4-benzyloxypent-2-enoate (6). This protected amino acid was used for the solid-phase peptide synthesis of oligopeptides, which serve as sequencespecific chromogenic protease substrates when used in the presence of NaIO 4 and bovine serum albumin. The peptide 1 (KRAVNleÀ5ÀEANleNH 2 (Nle norleucine)) allows detection of HIV-protease activity spectrophotometrically at 405 nm.

Who reads A Selective HIV-Protease Assay Based on a Chromogenic Amino Acid?

Author

Fabrizio Badalassi; Hong Khan Nguyen; Paolo Crotti; Jean-Louis Reymond

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0018-019X)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)