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Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device by Maria Maddalena Calabretta; Laura Montali; Antonia Lopreside; Fabio Fragapane; Francesco Iacoangeli; Aldo Roda; Valerio Bocci; Marcello D’Elia; Elisa Michelini is a Chemistry article available to read on EtoBox.
What is Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device about?
The availability of portable analytical devices for on-site monitoring and rapid detection of analytes of forensic, environmental, and clinical interest is vital. We report the development of a portable device for the detection of biochemiluminescence relying on silicon photomultiplier (SiPM) technology, called LuminoSiPM, which includes a 3D printed sample holder that can be adapted for both liquid samples and paper-based biosensing. We performed a comparison of analytical performance in terms of detectability with a benchtop luminometer, a portable cooled charge-coupled device (CCD sensor), and smartphone-integrated complementary metal oxide semiconductor (CMOS) sensors. As model systems, we used two luciferase/luciferin systems emitting at different wavelengths using purified protein solutions: the green-emitting __P. pyralis__ mutant Ppy-GR-TS (λ~max~ 550 nm) and the blue-emitting NanoLuc (λ~max~ 460 nm). A limit of detection of 9 femtomoles was obtained for NanoLuc luciferase, about 2 and 3 orders of magnitude lower than that obtained with the portable CCD camera and with the smartphone, respectively. A proof-of-principle forensic application of LuminoSiPM is provided, exploit
Who reads Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Maria Maddalena Calabretta; Laura Montali; Antonia Lopreside; Fabio Fragapane; Francesco Iacoangeli; Aldo Roda; Valerio Bocci; Marcello D’Elia; Elisa Michelini
- Publisher
- American Chemical Society (ACS)
- Published
- 2021
- Language
- EN
- Field
- Chemistry (Physical Sciences)