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Can I read Prevention of Mucosal Atrophy: Role of Glutamine and Caspases in Apoptosis in Intestinal Epithelial Cells, on EtoBox?
Prevention of Mucosal Atrophy: Role of Glutamine and Caspases in Apoptosis in Intestinal Epithelial Cells, by Harry T. Papaconstantinou; Dai H. Chung; Weiping Zhang; Naseem H. Ansari; Mark R. Hellmich; Courtney M. Townsend Jr.; Tien C. Ko is a Medicine article available to read on EtoBox.
What is Prevention of Mucosal Atrophy: Role of Glutamine and Caspases in Apoptosis in Intestinal Epithelial Cells, about?
Glutamine starvation induces apoptosis in enterocytes; therefore glutamine is important in the maintenance of gut mucosal homeostasis. However, the molecular mechanisms are unknown. The caspase family of proteases constitutes the molecular machinery that drives apoptosis. Caspases are selectively activated in a stimulus-specific and tissue-specific fashion. The aims of this study were to (1) identify specific caspases activated by glutamine starvation and (2) determine whether a general caspase inhibitor blocks glutamine starvation-induced apoptosis in intestinal epithelial cells. Rat intestinal epithelial (RIE-I) cells were deprived of glutamine. Specific caspase activation was measured using fluorogenic substrate assay. Apoptosis was quantified by DNA fragmentation and Hoechst nuclear staining. Glutamine starvation of RIE-1 cells resulted in the time-dependent activation of caspases 3 (10 hours) and 2 (18 hours), and the induction of DNA fragmentation (12 hours). Caspases 1 and 8 remained inactive. ZVAD-fluoromethyl ketone, a general caspase inhibitor, completely blocked glutamine starvation-induced caspase activation, DNA fragmentation, and nuclear condensation. These results in
Who reads Prevention of Mucosal Atrophy: Role of Glutamine and Caspases in Apoptosis in Intestinal Epithelial Cells,?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Harry T. Papaconstantinou; Dai H. Chung; Weiping Zhang; Naseem H. Ansari; Mark R. Hellmich; Courtney M. Townsend Jr.; Tien C. Ko
- Publisher
- Springer-Verlag; Springer Verlag; Elsevier BV; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 1091-255X)
- Published
- 2000
- Language
- EN
- Field
- Medicine (Health Sciences)