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Psoralen‐mediated Photodecontamination of Platelet Concentrates: Inactivation of Cell‐free and Cell‐associated Forms of Human Immunodeficiency Virus and Assessment of Platelet Function in Vivo by H. Margolis‐Nunno; L. Bardossy; R. Robinson; E. Ben‐Hur; B. Horowitz; M. A. Blajchman is a Medicine article available to read on EtoBox.
What is Psoralen‐mediated Photodecontamination of Platelet Concentrates: Inactivation of Cell‐free and Cell‐associated Forms of Human Immunodeficiency Virus and Assessment of Platelet Function in Vivo about?
BACKGROUND: Treatment of platelet concentrates (PCs) with psoralens and broad‐band ultraviolet A (UVA) radiation is being examined for the elimination of pathogens that might be present in donated blood. Previous studies have demonstrated the inactivation of cell‐free viruses and the maintenance of platelet integrity with common in vitro assays. STUDY DESIGN AND METHODS: Human immunodeficiency virus (HIV) in three forms‐cell‐free, activity replicating, and latently infected cell lines‐was added to PCs and treated with 50‐microgram per mL of 4′‐ aminomethyl‐4,5′,8‐trimethylpsoralen (AMT), 0.35 mM rutin, and broad‐ and narrow‐band UVA light (320‐400 nm and 360–370 nm [UVA1], respectively). The inactivation of added HIV was assessed in tissue culture; platelet hemostatic activity was assessed in thrombocytopenic rabbits. RESULTS: Each form of HIV was inactivated completely (> or = 10(5) infectious units) on treatment with 30 J per cm2 of UVA1 light. Similar results were obtained on treatment of 2.5 mL of PCs in test tubes or intact PC units (50 mL) in blood bags. Latently infected cell lines were substantially more sensitive than cell‐free HIV or HIV that was actively replicating. Hum
Who reads Psoralen‐mediated Photodecontamination of Platelet Concentrates: Inactivation of Cell‐free and Cell‐associated Forms of Human Immunodeficiency Virus and Assessment of Platelet Function in Vivo?
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- Author
- H. Margolis‐Nunno; L. Bardossy; R. Robinson; E. Ben‐Hur; B. Horowitz; M. A. Blajchman
- Publisher
- Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Health Sciences)