Can I read Macrophage/microglia Activation Factor Expression Is Restricted to Lesion‐associated Microglial Cells After Brain Trauma on EtoBox?

Macrophage/microglia Activation Factor Expression Is Restricted to Lesion‐associated Microglial Cells After Brain Trauma by Anna Lünemann; Oliver Ullrich; Antje Diestel; Thomas Jöns; Olaf Ninnemann; Adam Kovac; Elena E. Pohl; Ralf Hass; Robert Nitsch; Sven Hendrix is a Neuroscience article available to read on EtoBox.

What is Macrophage/microglia Activation Factor Expression Is Restricted to Lesion‐associated Microglial Cells After Brain Trauma about?

## Abstract After traumatic brain lesion, microglial cells are rapidly activated, migrate toward the sites of injury, and cause secondary damage that accounts for most of the loss of brain function. In the present study, we have characterized a new macrophage/microglia activation factor (MAF). Using the monocytic cell line U937, we were able to demonstrate that MAF is upregulated after TPA‐induced differentiation into macrophages. We have generated a specific antibody against MAF. In BV‐2 microglial cells, MAF is partially co‐localized with IB4, a classical microglial marker. In addition, we have analyzed the in vivo expression patterns of MAF after entorhinal cortex lesion. We were able to show a substantial upregulation of MAF on selected CD11b^+^ and IB4^+^ macrophages/microglial cells in the deafferented hippocampus and in the perilesional region, while no MAF expression was detectable on the contralateral side. Confocal microscopy revealed a lysosome‐like expression pattern in BV‐2 cells, as well as in ECL‐associated macrophages/microglial cells in vivo. Furthermore, we were able to demonstrate that U937 cells with downregulated MAF converted slower and to a significantly redu

Who reads Macrophage/microglia Activation Factor Expression Is Restricted to Lesion‐associated Microglial Cells After Brain Trauma?

Author

Anna Lünemann; Oliver Ullrich; Antje Diestel; Thomas Jöns; Olaf Ninnemann; Adam Kovac; Elena E. Pohl; Ralf Hass; Robert Nitsch; Sven Hendrix

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2005

Language

EN

Field

Neuroscience (Life Sciences)