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Examination of Ribosome-like Particles in Isolated Prolamellar Bodies by A. R. Wellburn; P. H. Quail; B. E. S. Gunning is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Examination of Ribosome-like Particles in Isolated Prolamellar Bodies about?

Potential methods for the preparation of fractions enriched in prolamellar bodies (PLBs) were examined in detail. Sucrose density gradient centrifugation methods gave fractions consisting almost exclusively of PLBs whilst those methods employing differential centrifugation were quite successful but contained greater quantities of lamellar membranes. Greater difficulty was experienced in obtaining detached PLBs which retained their "ribosome-like" lattice particles. No modification to density gradient procedures was found which retained these particles but the omission of ethylene diaminetetraacetic acid (EDTA) from all media including that of lysis gave a hint that this was possible with differential centrifugal methods. This was developed to produce a successful method for the preparation of PLBs which retain the "ribosome-like" particles of the lattice. Such fractions from Arena sativa L. and Hordeum vulgare L. were treated with ribonuclease which completely removed these particles from the lattice structures implying that they may be "ribosomal" in nature. EDTA apparently has a critical effect on PLB structure at concentration lower than those that effect the chloroplast couplin

Who reads Examination of Ribosome-like Particles in Isolated Prolamellar Bodies?

Author

A. R. Wellburn; P. H. Quail; B. E. S. Gunning

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1977

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)