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The Extent of Apoptosis Is Inversely Associated with Bcl-2 Expression in Premalignant and Malignant Breast Lesions by M. MUSTONEN; H. RAUNIO; P. PÄÄKKÖ; Y. SOINI is a Medicine article available to read on EtoBox.

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## Aims: In this study we investigated the extent of apoptosis in benign, premalignant and malignant breast lesions and its association with the immunohistochemical expression of bcl-2 oncoprotein. ## Methods and results: In order to detect apoptotic cells and bodies in tissue sections, the 3'-end dna labelling method was used. immunohistochemical staining was performed by using the avidin-biotin-peroxidase complex technique. a monoclonal antibody against bcl-2 oncoprotein was used and the specificity of the antibody was confirmed by immunoblot analysis. according to the results the extent of apoptosis, as determined by the apoptotic index, was lowest in benign ductal hyperplasias and sclerosing adenoses (0.15% and 0.07%, respectively). it was moderately elevated in atypical hyperplasias and in-situ carcinomas (0.20% and 0.40%, respectively) and highest in invasive carcinomas (0.76%). in ductal invasive carcinomas, grade i lesions showed a lower apoptotic index (0.52%) than grade ii (0.72%) and grade iii (1.17%) carcinomas. the apoptotic index was not significantly lower in lobular (0.82%) than in ductal invasive carcinomas (0.85%). bcl-2 immunohistochemistry was inversely related

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Author

M. MUSTONEN; H. RAUNIO; P. PÄÄKKÖ; Y. SOINI

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0309-0167)

Published

1997

Language

EN

Field

Medicine (Health Sciences)