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Apoptosis Induced via AMPA-Selective Glutamate Receptors in Cultured Murine Cortical Neurons by Jari A. Larm; Nam S. Cheung; Philip M. Beart is a Neuroscience article available to read on EtoBox.

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We have investigated the mechanisms of cell death induced by long-term exposure to the glutamate receptor agonist (S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate [(S)-AMPA]. Using primary cultures of pure neurons (95%) grown in serum-free conditions, we found that 24-h exposure to (S)-AMPA (0.01-1,000 microM) induced concentration-dependent neuronal cell death (EC50 = 3 +/- 0.5 microM) with cellular changes including neurite blebbing, chromatin condensation, and DNA fragmentation, indicative of apoptosis. (S)-AMPA induced a delayed cell death with DNA fragmentation occurring in approximately 50% of cells at concentrations between 100 and 300 microM detected using terminal transferase-mediated dUTP nick end-labeling (TUNEL) and agarose gel electrophoresis. Apoptotic chromatin condensation was detected using 4,6-diamidino-2-phenylindole, a fluorescent DNA binding dye. Cell death induced by (S)-AMPA was attenuated by the AMPA receptor-selective antagonist LY293558 (10 microM) and the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 50 microM), yielding EC50 values of 73 +/- 5 and 265 +/- 8 microM, respectively, and was unaffected by the NMDA receptor a

Who reads Apoptosis Induced via AMPA-Selective Glutamate Receptors in Cultured Murine Cortical Neurons?

Author

Jari A. Larm; Nam S. Cheung; Philip M. Beart

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)