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Metabolic flux regulates growth transitions and antibiotic tolerance in uropathogenic Escherichia coli by Josiah J. Morrison; Daniel A. Banas; Ellen K. Madden; Eric C. DiBiasio; David C. Rowley; Paul S Cohen; Jodi L. Camberg is a scholarly article available to read on EtoBox.

What is Metabolic flux regulates growth transitions and antibiotic tolerance in uropathogenic Escherichia coli about?

Reducing growth and limiting metabolism are strategies that allow bacteria to survive exposure to environmental stress and antibiotics. During infection, uropathogenic __Escherichia coli__ (UPEC) may enter a quiescent state that enables them to reemerge after completion of successful antibiotic treatment. Many clinical isolates, including the well characterized UPEC strain CFT073, also enter a metabolite-dependent, quiescent state in vitro that is reversible with cues, including peptidoglycan-derived peptides and amino acids. Here, we show that quiescent UPEC is antibiotic tolerant and demonstrate that metabolic flux in the tricarboxylic acid (TCA) cycle regulates the UPEC quiescent state via succinyl-CoA. We also demonstrate that the transcriptional regulator complex IHF and the FtsZ-interacting protein ZapE, which is important for __E. coli__ division during stress, are essential for UPEC to enter the quiescent state. Notably, in addition to engaging FtsZ and late-stage cell division proteins, ZapE also interacts directly with TCA cycle enzymes in bacterial two hybrid assays. We report direct interactions between succinate dehydrogenase complex subunit SdhC, the late-stage cell d

Author

Josiah J. Morrison; Daniel A. Banas; Ellen K. Madden; Eric C. DiBiasio; David C. Rowley; Paul S Cohen; Jodi L. Camberg

Publisher

Cold Spring Harbor Laboratory

Published

2023

Language

EN