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Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation by Hikari Nishisaka; Takumi Tomohiro; Kako Fukuzumi; Akira Fukao; Yoshinori Funakami; Toshinobu Fujiwara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation about?

The RNA-binding protein HuD/ELAVL4 is essential for neuronal development and synaptic plasticity by governing various post-transcriptional processes of target mRNAs, including stability, translation, and localization. We previously showed that the linker region and poly(A)-binding domain of HuD play a pivotal role in promoting translation and inducing neurite outgrowth. In addition, we found that HuD interacts exclusively with the active form of Akt1, through the linker region. Although this interaction is essential for neurite outgrowth, HuD is not a substrate for Akt1, raising questions about the dynamics between HuD-mediated translational stimulation and its association with active Akt1. Here, we demonstrate that active Akt1 interacts with the cap-binding complex via HuD. We identify key amino acids in linker region of HuD responsible for Akt1 interaction, leading to the generation of two point-mutated HuD variants: one that is incapable of binding to Akt1 and another that can interact with Akt1 regardless of its phosphorylation status. In vitro translation assays using these mutants reveal that HuD-mediated translation stimulation is independent of its binding to Akt1. In addit

Who reads Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation?

Author

Hikari Nishisaka; Takumi Tomohiro; Kako Fukuzumi; Akira Fukao; Yoshinori Funakami; Toshinobu Fujiwara

Publisher

Elsevier BV

Published

2024

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)