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The potential diagnostic use of K-ras codon 12 and p53 alterations in brush cytology from the pancreatic head region by Sturm, Patrick D. J.; Hruban, Ralph H.; Ramsoekh, Teun B.; Noorduyn, L. Arnold; Tytgat, Guido N. J.; Gouma, Derk J.; Offerhaus, G. Johan A. is a Medicine article available to read on EtoBox.

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It can be difficult to distinguish between malignant and benign disease of the region of the head of the pancreas using conventional methods. K-ras and p53 alterations occur frequently in malignancies in this region and are therefore candidate tumour markers. To define the utility of these alterations in interpreting pancreatic head cytology, the present study investigated to what extent alterations in the carcinomas were detectable on cytology and whether the alterations found in the cytology came from the carcinomas. Fifty-seven consecutive pancreaticoduodenectomy resection specimens (52 with a malignancy and five without) and the ductal brush cytology specimens collected post-operatively from these resection specimens were compared for the presence of K-ras and p53 alterations. K-ras mutations were detected using the polymerase chain reaction (PCR) and allele-specific oligonucleotide hybridization, and p53 alterations using immunochemical staining for the p53 gene product. After discrepancy analysis, the results from the resection specimens and corresponding brush cytology specimens were identical in 88 per cent for the K-ras analysis and in 84 per cent for the p53 analysis. In

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Author

Sturm, Patrick D. J.; Hruban, Ralph H.; Ramsoekh, Teun B.; Noorduyn, L. Arnold; Tytgat, Guido N. J.; Gouma, Derk J.; Offerhaus, G. Johan A.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

1999

Language

EN

Field

Medicine (Health Sciences)