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Calcium-Mediated Degeneration of the Axonal Cytoskeleton in the Ola Mouse by Jonathan D. Glass; Brenda L. Schryer; John W. Griffin is a Neuroscience article available to read on EtoBox.

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Abstract: The C57BL/Ola (Ola) mouse is a mutant substrain in which transected axons undergo very slow Wallerian degeneration. Because axonal degradation during Wallerian degeneration is calcium dependent, we tested whether Ola axons are susceptible to calcium‐mediated axonal degeneration by comparing neuro‐filament degradation between Ola and C57BL/6 mice in sciatic nerve explants. Using immunoblot analysis of neurofilament degradation and electron microscopy we found that as in normal axons, axonal degeneration in the Ola is calcium dependent. However, when compared with normal animals, higher levels of calcium were required for complete degradation of neurofilaments in Ola nerve, suggesting a relative insensitivity to calcium‐mediated degeneration in the Ola. We conclude that calcium‐activated proteases are present and active in Ola axons but that higher levels of calcium are required to accomplish complete axonal degradation. These results suggest a possible mechanism for prolonged survival of transected Ola axons and provide potential insight into the pathophysiology of axonal degeneration in injury and disease.

Who reads Calcium-Mediated Degeneration of the Axonal Cytoskeleton in the Ola Mouse?

Author

Jonathan D. Glass; Brenda L. Schryer; John W. Griffin

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1994

Language

EN

Field

Neuroscience (Life Sciences)