Can I read Effect of Neostigmine on Concentration and Extraction Fraction of Acetylcholine Using Quantitative Microdialysis on EtoBox?

Effect of Neostigmine on Concentration and Extraction Fraction of Acetylcholine Using Quantitative Microdialysis by P.N Vinson; J.B Justice Jr. is a Neuroscience article available to read on EtoBox.

What is Effect of Neostigmine on Concentration and Extraction Fraction of Acetylcholine Using Quantitative Microdialysis about?

A quantitative microdialysis method was used to determine the effect of local perfusion of 0, 100, 200, and 300 nM neostigmine (NEO) on acetylcholine (ACh) extracellular concentration and microdialysis extraction fraction (E d ) in the striatum of the rat. Because of the efficiency of AChE, the inhibition of this enzyme is expected to result in a substantial increase in ACh levels and a decrease in the E d of ACh. The extracellular concentration of ACh increased linearly with increasing concentrations of NEO. The control ACh concentration was determined to be 18.4 9 11.8 nM (n= 10; mean9 S.E.M.) The ACh extracellular concentration for the remaining groups was determined to be 1739 14 nM (n= 5), 3299 52.5 nM (n= 13), and 5819 109 nM (n =10) for the 100, 200, and 300 nM NEO groups, respectively. Perfusion with 300 nM NEO resulted in a significant reduction in the E d of ACh (64.59 3.5% vs. 43.6 97.5%, P B0.05). In contrast to ACh, perfusion with 0, 1, and 10 vM hemicholinium-3, an inhibitor of high-affinity choline uptake, increased choline levels but did not affect the E d of choline. The effects on E d are consistent with E d being influenced by rapid clearance mechanisms.

Who reads Effect of Neostigmine on Concentration and Extraction Fraction of Acetylcholine Using Quantitative Microdialysis?

Author

P.N Vinson; J.B Justice Jr.

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)