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Induction of Glucose-6-Phosphate Dehydrogenase by Lipopolysaccharide Contributes to Preventing Nitric Oxide-Mediated Glutathione Depletion in Cultured Rat Astrocytes by Paula García-Nogales; Angeles Almeida; Emilio Fernández; José M. Medina; Juan P. Bolaños is a Neuroscience article available to read on EtoBox.
What is Induction of Glucose-6-Phosphate Dehydrogenase by Lipopolysaccharide Contributes to Preventing Nitric Oxide-Mediated Glutathione Depletion in Cultured Rat Astrocytes about?
Abstract: Treatment of cultured rat astrocytes with lipopolysaccharide (LPS; 1 μg/ml) increased mRNA expression of glucose‐6‐phosphate dehydrogenase (G6PD), the rate‐limiting step in the pentose phosphate pathway (PPP), in a time‐dependent fashion (0‐24 h). This effect was accompanied by an increase in G6PD activity (1.74‐fold) and in the rate of glucose oxidation through the PPP (6.32‐fold). Inhibition of inducible nitric oxide synthase (iNOS) activity by 2‐amino‐5,6‐dihydro‐6‐methyl‐4__H__‐1,3‐thiazine (AMT; 50 μ__M__) did not alter the LPS‐mediated enhancement of G6PD mRNA expression or PPP activity. Blockade of nuclear factor κB (NF‐κB) activation by __N__‐benzyloxycarbonyl‐Ile‐Glu‐(__O‐tert__‐butyl)‐Ala‐leucinal (1 μ__M__) prevented the expression of both iNOS mRNA and G6PD mRNA, suggesting that iNOS and G6PD are co‐induced by LPS through a common transcriptional pathway involving NF‐κB activation. Incubation of cells with LPS for 24 h increased intracellular NADPH concentrations (1.63‐fold) as compared with untreated cells, but GSH concentrations were not modified by LPS treatment up to 60 h of incubation. However, inhibition of G6PD activity by dehydroepiandrosterone (DHEA;
Who reads Induction of Glucose-6-Phosphate Dehydrogenase by Lipopolysaccharide Contributes to Preventing Nitric Oxide-Mediated Glutathione Depletion in Cultured Rat Astrocytes?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Paula García-Nogales; Angeles Almeida; Emilio Fernández; José M. Medina; Juan P. Bolaños
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1999
- Language
- EN
- Field
- Neuroscience (Life Sciences)