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Rapid method for purification of Clostridium botulinuh type C neurotoxin by High Performance Liquid Chromatography (HPLC ) by M. Matsuda; K. Ozutsumi; Du Pei-Ying; N. Sugimoto is a Medicine article available to read on EtoBox.

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The culture supernatant of CIostridium botulinum ,type C, concentrated by addition of RNA, acid precipitation and subsequent protamine treatment yeas used as starting material for rapid purification of L toxin (mol. wt. ca. 50OK) and M toxin (mol. wt. ca. 350K) of C1 neurotoxin by ion-exchange chromatography on a Mono S column by fast performance liquid chromatography (FPLC). L and M toxins were highly purified further by gel permeation chromatography through a TSK G3009SW column at pH 6.0 by high performance liquid chromatography (HPLC). Purified S toxin (mol. wt. ca. 150K, C1 neurotoxin without a nontoxic component) was then obtained from L toxin rapidly by gel permeation chromatography at pH 7.3 through a TSK G3000SW column by HPLC. Pui-ified S toxin was also obtained rapidly from M and L toxins by ion-exchange chromatography on a Mono Q column at pH 8.0 using an FPLC system. 1"he purified preparations of L, M and S toxins gave single bands on conventional polyacrylamide gel electrophoresis, and had specific activities of 2.8, 6.7, and 14-21 × 107 LD~0/mg N, respectively, in mice. On immunoelectrophoresis, purified S toxin gave a single arc against anti-crude toxin serum. The yi

Who reads Rapid method for purification of Clostridium botulinuh type C neurotoxin by High Performance Liquid Chromatography (HPLC )?

Author

M. Matsuda; K. Ozutsumi; Du Pei-Ying; N. Sugimoto

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0393-2990)

Published

1986

Language

EN

Field

Medicine (Health Sciences)