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Can I read Sperm Chromatin Structure and Reproductive Fitness Are Altered by Substitution of a Single Amino Acid in Mouse Protamine 1 on EtoBox?
Sperm Chromatin Structure and Reproductive Fitness Are Altered by Substitution of a Single Amino Acid in Mouse Protamine 1 by Lindsay Moritz; Samantha B. Schon; Mashiat Rabbani; Yi Sheng; Ritvija Agrawal; Juniper Glass-Klaiber; Caleb Sultan; Jeannie M. Camarillo; Jourdan Clements; Michael R. Baldwin; Adam G. Diehl; Alan P. Boyle; Patrick J. O’Brien; Kaushik Ragunathan; Yueh-Chiang Hu; Neil L. Kelleher; Jayakrishnan Nandakumar; Jun Z. Li; Kyle E. Orwig; Sy Redding; Saher Sue Hammoud is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sperm Chromatin Structure and Reproductive Fitness Are Altered by Substitution of a Single Amino Acid in Mouse Protamine 1 about?
Conventional dogma presumes that protamine-mediated DNA compaction in sperm is achieved by electrostatic interactions between DNA and the arginine-rich core of protamines. Phylogenetic analysis reveals several non-arginine residues conserved within, but not across species. The significance of these residues and their post-translational modifications are poorly understood. Here, we investigated the role of K49, a rodent-specific lysine residue in protamine 1 (P1) that is acetylated early in spermiogenesis and retained in sperm. In sperm, alanine substitution (P1(K49A)) decreases sperm motility and male fertility-defects that are not rescued by arginine substitution (P1(K49R)). In zygotes, P1(K49A) leads to premature male pronuclear decompaction, altered DNA replication, and embryonic arrest. In vitro, P1(K49A) decreases protamine-DNA binding and alters DNA compaction and decompaction kinetics. Hence, a single amino acid substitution outside the P1 arginine core is sufficient to profoundly alter protein function and developmental outcomes, suggesting that protamine non-arginine residues are essential for reproductive fitness. Spermatogenesis is a highly regulated differentiation proc
Who reads Sperm Chromatin Structure and Reproductive Fitness Are Altered by Substitution of a Single Amino Acid in Mouse Protamine 1?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Lindsay Moritz; Samantha B. Schon; Mashiat Rabbani; Yi Sheng; Ritvija Agrawal; Juniper Glass-Klaiber; Caleb Sultan; Jeannie M. Camarillo; Jourdan Clements; Michael R. Baldwin; Adam G. Diehl; Alan P. Boyle; Patrick J. O’Brien; Kaushik Ragunathan; Yueh-Chiang Hu; Neil L. Kelleher; Jayakrishnan Nandakumar; Jun Z. Li; Kyle E. Orwig; Sy Redding; Saher Sue Hammoud
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)