Can I read Aromatic and pyrone polyketides synthesized by a stilbene synthase from Rheum tataricum☆☆The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL/DDBJ with the accession number AF508150. on EtoBox?

Aromatic and pyrone polyketides synthesized by a stilbene synthase from Rheum tataricum☆☆The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL/DDBJ with the accession number AF508150. by Supachai Samappito; Jonathan E Page; Jürgen Schmidt; Wanchai De-Eknamkul; Toni M Kutchan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Aromatic and pyrone polyketides synthesized by a stilbene synthase from Rheum tataricum☆☆The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL/DDBJ with the accession number AF508150. about?

A cDNA encoding a stilbene synthase, RtSTS, was isolated from the rhizomes of Tatar rhubarb, Rheum tataricum L. (Polygonaceae), a medicinal plant containing stilbenes and other polyketides. Recombinant RtSTS was expressed in E. coli and assayed with acetyl-coenzyme A (CoA), n-butyryl-CoA, isovaleryl-CoA, n-hexanoyl-CoA, cinnamoyl-CoA and p-coumaroyl-CoA as primers of polyketide synthesis. RtSTS synthesized resveratrol and a trace amount of naringenin chalcone from p-coumaroyl-CoA, supporting the enzyme's identification as a resveratrol-type stilbene synthase (EC 2.3.1.95). Bis-noryangonin and p-coumaroyl triacetic acid lactone (CTAL)-type pyrones were observed in minor amounts in the reaction with p-coumaroyl-CoA and as major products with cinnamoyl CoA. As well, such pyrones, and not aromatic polyketides, were identified as the only products in assays with aliphatic and benzoyl CoA esters. Acetonyl-4-hydroxy-2-pyrone, a pyrone synthesized from acetyl-CoA, was identified as a new product of a stilbene synthase. Using Northern blot analysis, RtSTS transcript was found to be highly expressed in R. tataricum rhizomes, with low transcript levels also present in young leaves. This expre

Who reads Aromatic and pyrone polyketides synthesized by a stilbene synthase from Rheum tataricum☆☆The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL/DDBJ with the accession number AF508150.?

Author

Supachai Samappito; Jonathan E Page; Jürgen Schmidt; Wanchai De-Eknamkul; Toni M Kutchan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)