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Can I read Cell‐cycle modulation of CK2 activity in tobacco BY‐2 cells^†^ on EtoBox?
Cell‐cycle modulation of CK2 activity in tobacco BY‐2 cells^†^ by M. Carme Espunya; Bruno Combettes; Josep Dot; Nicole Chaubet‐Gigot; M. Carmen Martínez is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Cell‐cycle modulation of CK2 activity in tobacco BY‐2 cells^†^ about?
## Summary Protein kinase CK2 is an ubiquitous Ser/Thr kinase essential for cell growth. We have used the highly synchronizable tobacco BY‐2 cell line to investigate whether CK2 activity and expression are regulated in a cell cycle phase‐dependent manner in higher plants. Specific cDNA probes for tobacco CK2α and β subunits, respectively, and polyclonal antibodies recognising α and β subunits separately, were obtained to determine mRNA and protein levels of both subunits. Our results show that CK2 activity oscillates throughout the cell cycle, peaking at G1/S and M phases, due to a post‐translational regulation of the tetrameric enzyme. Additional levels of control of CK2 expression operate in relation to the proliferative state of the cells, including differential accumulation of α and β transcripts and post‐transcriptional regulation of protein levels (β subunit). Moreover, __in vivo__ inhibition of CK2 activity corroborates the requirement of the functional CK2 to progress through the cell division cycle, and suggests that CK2 might play an important role at the G2/M checkpoint.
Who reads Cell‐cycle modulation of CK2 activity in tobacco BY‐2 cells^†^?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- M. Carme Espunya; Bruno Combettes; Josep Dot; Nicole Chaubet‐Gigot; M. Carmen Martínez
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)
- Published
- 1999
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)