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New Colorimetric Substrates for the Assay of Glycosidases by Kai H. Aamlid; Grantham Lee; Brian V. Smith; Anthony C. Richardson; Robert G. Price is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is New Colorimetric Substrates for the Assay of Glycosidases about?

The preferred method of detecting and assaying enzymes is to incubate with a substance which is as close to the natural substrate as possible, but which will liberate a molecule that can be quantified on the basis of its colour, fluorescence, or chemiluminescence. The last two properties are particularly sensitive, but their measurement requires specialised equipment. The most widely used substrates for the assay of glycosides are the 4-and 2nitrophenyl glycosides, but the nitrophenols released, after basification, have weak yellow colours (A,, -410 nm) and endogenous coloured species may interfere in the assays. Appropriate derivatives of the highly coloured 2-methoxy-4-(2nitrovinyl)phenol' (2a; Amax 505, E 27 000) are good substrates for the calorimetric assay of glycosidas-es2, phosphatases, and esterases3. However, the colour of the phenol released tends to fade under alkaline conditions~ and the substrates are not as soluble in water as is required for general use. The condensation of vanillyl2-acetamido-2-deoxy-/I-D-glucopyranoside (lb) and vanillyl j?-D-galactopyranoside (lc) with various heterocycles having either an active methyl or methylene group has now been studied in

Who reads New Colorimetric Substrates for the Assay of Glycosidases?

Author

Kai H. Aamlid; Grantham Lee; Brian V. Smith; Anthony C. Richardson; Robert G. Price

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)