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Interferon-α–inducible Proteins Are Novel Autoantigens in Murine Lupus by Wolfgang Hueber; Defu Zeng; Samuel Strober; Paul J. Utz is a Medicine article available to read on EtoBox.

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## Abstract ## Objective To investigate the spectrum of B cell autoimmunity in the recently described anti‐CD1–autoreactive T cell receptor (TCR)–transgenic murine lupus‐like (CD1 lupus‐like) model. ## Methods Lethally irradiated BALB/c/nu/nu mice were injected intravenously with donor BALB/c bone marrow and spleen cells expressing TCRα and TCRβ transgenes that recognize CD1d. Sera from adoptive host animals that developed lupus (i.e., CD1 lupus mice) were collected at serial time points and analyzed by Western blotting and immunoprecipitation, using protein extracts prepared from NIH3T3 mouse fibroblasts and EL‐4 lymphocytes, respectively. Sera obtained from older animals in several models of spontaneous lupus (NZB/NZW, MRL++, and MRL/__lpr__ mice), unmanipulated BALB/c/nu/nu mice, and normal BALB/c mice were used as controls. ## Results Analyses demonstrated that the prominent targets of autoantibodies in the CD1 lupus‐like model are interferon‐α (IFNα)–inducible antigens. Biochemical and serologic characterizations identified one antigen as belonging to the interferon‐inducible 202 (Ifi202) subfamily of proteins within the Ifi200 family, and a second antigen as a member of the 7

Who reads Interferon-α–inducible Proteins Are Novel Autoantigens in Murine Lupus?

Author

Wolfgang Hueber; Defu Zeng; Samuel Strober; Paul J. Utz

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2004

Language

EN

Field

Medicine (Health Sciences)