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Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: Thermodynamic and crystallographic studies by Evangelia G. Kapetaniou; Angelos Thanassoulas; Anatoly P. Dubnovitsky; George Nounesis; Anastassios C. Papageorgiou is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: Thermodynamic and crystallographic studies about?
## Abstract pH is one of the key parameters that affect the stability and function of proteins. We have studied the effect of pH on the pyridoxal‐5′‐phosphate‐dependent enzyme phosphoserine aminotransferase produced by the facultative alkaliphile __Bacillus circulans__ ssp. __alkalophilus__ using thermodynamic and crystallographic analysis. Enzymatic activity assay showed that the enzyme has maximum activity at pH 9.0 and relative activity less than 10% at pH 7.0. Differential scanning calorimetry and circular dichroism experiments revealed variations in the stability and denaturation profiles of the enzyme at different pHs. Most importantly, release of pyridoxal‐5′‐phosphate and protein thermal denaturation were found to occur simultaneously at pH 6.0 in contrast to pH 8.5 where denaturation preceded cofactor's release by approximately 3°C. To correlate the observed differences in thermal denaturation with structural features, the crystal structure of phosphoserine aminotransferase was determined at 1.2 and 1.5 Å resolution at two different pHs (8.5 and 4.6, respectively). Analysis of the two structures revealed changes in the vicinity of the active site and in surface residues. A
Who reads Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: Thermodynamic and crystallographic studies?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Evangelia G. Kapetaniou; Angelos Thanassoulas; Anatoly P. Dubnovitsky; George Nounesis; Anastassios C. Papageorgiou
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)