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In vitro modulation of L‐arginine transport in trophoblast cells by glucose by Eaton; Sooranna is a Medicine article available to read on EtoBox.

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## Background The uptake of the semi–essential amino acid, L‐arginine, into trophoblast cells was measured with the aim of determining the effect of different glucose concentrations on L‐arginine influx kinetics. ## Methods This study used a novel superfused microcarrier culture system of BeWo cells (an established choriocarcinoma cell line with many characteristics of normal human trophoblast) and a rapid, paired‐tracer dilution technique to measure unidirectional influx into the cells. ## Results At 10 mmol L^−1^D‐glucose, L‐arginine unidirectional influx across the microvillous border of the cells was saturable with a __K__~m~ of 1.14 ± 0.14 mmol L^−1^ and a __V__~max~ of 121.36 ± 5.89 nmol mg^−1^ protein min^−1^. When cells were preincubated for 24 h in the presence of 30 mmol L^−1^D‐glucose, there was a significant increase in the __V__~max~ for L‐arginine of nearly 30%. Similarly, preincubation in the presence of 1 mmol L^−1^D‐glucose and 12.5 mIU mL^−1^ human insulin reduced the __K__~m~ for L‐arginine influx by over 55%. ## Conclusion These data suggest that the modulation of placental transport of L‐arginine by glucose and insulin could contribute to the fetal macrosomia o

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Author

Eaton; Sooranna

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0014-2972)

Published

1998

Language

EN

Field

Medicine (Health Sciences)