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Polymorphonuclear Cell Apoptosis in Exudates Generated by Polymers by T. Fabre; F. Belloc; B. Dupuy; M. Schappacher; A. Soum; J. Bertrand-Barat; C. Baquey; A. Durandeau is a Medicine article available to read on EtoBox.

What is Polymorphonuclear Cell Apoptosis in Exudates Generated by Polymers about?

Flow cytometry was used to quantify apoptotic and necrotic polymorphonuclear (PMN) cells in an exudate generated by biomaterials, and the results were compared with determinations of spontaneous apoptosis and necrosis in PMN cells from the bloodstream. The exudate formed inside cylindrical tubes subcutaneously implanted in the dorsal region of rats was collected over a 1-week period. A rapid and simple staining procedure based on the spectral properties of the bisbenzemide Hoechst 33342 was used to identify apoptotic PMN cells. Quantification of permeabilized PMN cells stained by propidium iodide was possible in the same unfixed specimens. The percentages of apoptotic and permeabilized PMN cells in peripheral rat blood were low (1.8 ± 0.5% and 1.7 ± 0.7%, respectively), similar to results found in humans. In exudates generated by polyvinyl chloride (PVC), the percentages of apoptotic and permeabilized PMN cells were higher than in the blood. The percentage of PMN cells undergoing apoptosis progressively increased with time and reached a maximum at day 2 (27% ± 6%). The percentage of permeabilized cells progressively increased with time and was much higher than the percentage of apo

Who reads Polymorphonuclear Cell Apoptosis in Exudates Generated by Polymers?

Author

T. Fabre; F. Belloc; B. Dupuy; M. Schappacher; A. Soum; J. Bertrand-Barat; C. Baquey; A. Durandeau

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)

Published

1999

Language

EN

Field

Medicine (Health Sciences)