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Targeted Quantitative Bioanalysis in Plasma Using Liquid Chromatography/high‐resolution Accurate Mass Spectrometry: an Evaluation of Global Selectivity as a Function of Mass Resolving Power and Extraction Window, with Comparison of Centroid and Profile Modes by Yuan‐Qing Xia; Jim Lau; Timothy Olah; Mohammed Jemal is a Chemistry article available to read on EtoBox.

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There is a growing interest in exploring the use of liquid chromatography coupled with full‐scan high resolution accurate mass spectrometry (LC/HRMS) in bioanalytical laboratories as an alternative to the current practice of using LC coupled with tandem mass spectrometry (LC/MS/MS). Therefore, we have investigated the theoretical and practical aspects of LC/HRMS as it relates to the quantitation of drugs in plasma, which is the most commonly used matrix in pharmacokinetics studies. In order to assess the overall selectivity of HRMS, we evaluated the potential interferences from endogenous plasma components by analyzing acetonitrile‐precipitated blank human plasma extract using an LC/HRMS system under chromatographic conditions typically used for LC/MS/MS bioanalysis with the acquisition of total ion chromatograms (TICs) using 10 k and 20 k resolving power in both profile and centroid modes. From each TIC, we generated extracted ion chromatograms (EICs) of the exact masses of the [M + H]^+^ ions of 153 model drugs using different mass extraction windows (MEWs) and determined the number of plasma endogenous peaks detected in each EIC. Fewer endogenous peaks are detected using higher

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Author

Yuan‐Qing Xia; Jim Lau; Timothy Olah; Mohammed Jemal

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)