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Development of an antibiotics delivery system for topical treatment of the neglected tropical disease Buruli ulcer 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Preparation of PHBV microparticles 2 2.3 Preparation of GG-based spongy-like hydrogels 2 2.4 Physicochemical characterization of PHBV microparticles 2 2.4.1 Morphology and particle size distribution 2 2.4.2 Determination of encapsulation efficiency of RIF-loaded PHBV microparticles 3 2.4.3 Fourier transform Infrared Spectroscopy-Attenuated total reflectance (FTIR-ATR) 3 2.4.4 X-Ray diffractometry (XRD) 3 2.5 Characterization of GG-based spongy-like hydrogels 3 2.5.1 Morphological analysis 3 2.5.2 FTIR-ATR 3 2.5.3 Water uptake capacity 3 2.6 In vitro antibiotics release studies 3 2.6.1 Mathematical modelling of antibiotics release 3 2.7 In vitro antimicrobial activity studies 3 2.8 In vitro cytocompatibility studies 4 2.9 Statistical analysis 4 3 Results 5 3.1 Physical and chemical characterization of RIF-loaded PHBV microparticles 5 3.2 Characterization of GG-based spongy-like hydrogel loading RIF-loaded PHBV microparticles and STR 5 3.3 Antibacterial activity and cytocompatibility of the DDS 6 4 Discussi

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Language

UND

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nonfiction