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Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds by Rosa Lönneborg; Peter Brzezinski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds about?
## Background The transcriptional regulators DntR, NagR and NtdR have a high sequence identity and belong to the large family of LysR type transcriptional regulators (LTTRs). These three regulators are all involved in regulation of genes identified in pathways for degradation of aromatic compounds. They activate the transcription of these genes in the presence of an inducer, but the inducer specificity profiles are different. ## Results The results from this study show that NtdR has the broadest inducer specificity, responding to several nitro-aromatic compounds. Mutational studies of residues that differ between DntR, NagR and NtdR suggest that a number of specific residues are involved in the broader inducer specificity of NtdR when compared to DntR and NagR. The inducer response was also investigated as a function of the experimental conditions and a number of parameters such as the growth media, plasmid arrangement of the LTTR-encoding genes, promoter and __gfp__ reporter gene, and the presence of a His~6~-tag were shown to affect the inducer response in __E.coli__ DH5α. Furthermore, the response upon addition of both salicylate and 4-nitrobenzoate to the growth media was large
Who reads Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rosa Lönneborg; Peter Brzezinski
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central; Springer Science and Business Media LLC (ISSN 1471-2091)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)