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Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae by Ling Ye; Jan A. Berden; Karel van Dam; Arthur L. Kruckeberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract High‐affinity hexose transport is required for efficient utilization of low hexose concentrations by the baker's yeast __Saccharomyces cerevisiae__. These low concentrations occur during the late exponential phase of batch growth on hexoses, during hexose‐limited chemostat or fed‐batch culture, or during growth on sugars such as sucrose and raffinose that are hydrolysed to hexoses outside the cell. The expression of the Hxt7 high‐affinity glucose transporter of __S. cerevisiae__ was examined during batch growth on glucose medium in a wild‐type strain and a strain expressing only __HXT7__ (i.e. with null mutations in __HXT1–HXT6__). In the wild‐type strain, __HXT7__ transcription was repressed at high glucose and was detected when the glucose in the culture approached depletion. In the __HXT7__‐only strain, transcription of __HXT7__ was constitutive throughout the glucose growth phase and was increased further at low glucose concentrations. After glucose depletion, the levels of __HXT7__ mRNA declined rapidly in both strains. In contrast, the Hxt7 protein was relatively stable after glucose depletion. By monitoring the subcellular localization of an Hxt7::GFP fusion prot

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Author

Ling Ye; Jan A. Berden; Karel van Dam; Arthur L. Kruckeberg

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)