Can I read Determination of Endogenous Levels of 13-Cis-retinoic Acid (Isotretinoin), All-trans-retinoic Acid (Tretinoin) and Their 4-Oxo Metabolites in Human and Animal Plasma by High-performance Liquid Chromatography with Automated Column Switching and Ultraviolet Detection on EtoBox?
Determination of Endogenous Levels of 13-Cis-retinoic Acid (Isotretinoin), All-trans-retinoic Acid (Tretinoin) and Their 4-Oxo Metabolites in Human and Animal Plasma by High-performance Liquid Chromatography with Automated Column Switching and Ultraviolet Detection by Ronald Wyss; Franz Bucheli is a Chemistry article available to read on EtoBox.
What is Determination of Endogenous Levels of 13-Cis-retinoic Acid (Isotretinoin), All-trans-retinoic Acid (Tretinoin) and Their 4-Oxo Metabolites in Human and Animal Plasma by High-performance Liquid Chromatography with Automated Column Switching and Ultraviolet Detection about?
A highly sensitive HPLC method with automated column switching was developed for the simultaneous determination of endogenous levels of 13-cis-retinoic acid (isotretinoin), all-trans-retinoic acid (tretinoin) and their 4-oxo metabolites in plasma samples from man, Cynomolgus monkey, rabbit, rat and mouse. Plasma (0.4 ml) was deproteinated by adding ethanol (1.5 ml) containing the internal standard acitretin. After centrifugation, 1.4 ml of the supernatant were directly injected onto the precolumn packed with LiChrospher 100 RP-18 (5 microm). 1.25% ammonium acetate and acetic acid-ethanol (8:2, v/v) was used as mobile phase during injection and 1% ammonium acetate and 2% acetic acid-ethanol (102:4, v/v) was added, on-line, to decrease the elution strength of the injection solution. After backflush purging of the precolumn, the retained components were transferred to the analytical column in the backflush mode, separated by gradient elution and detected at 360 nm. Two coupled Superspher 100 RP-18 endcapped columns (both 250x4 mm) were used for the separation, together with a mobile phase consisting of acetonitrile-water-10% ammonium acetate-acetic acid: (A) 600:300:60:10 (v/v/v/v), (
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- Author
- Ronald Wyss; Franz Bucheli
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)