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Alpha-tocopherol Modulates Genes Involved in Hepatic Xenobiotic Pathways in Mice by Debbie J. Mustacich; Kishorchandra Gohil; Richard S. Bruno; Michelle Yan; Scott W. Leonard; Emily Ho; Carroll E. Cross; Maret G. Traber is a Medicine article available to read on EtoBox.

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Hepatic proteins involved in xenobiotic pathways (Phases I, II and III) are responsible for the metabolism and disposition of endogenous and exogenous compounds including dietary phytochemicals. To test the hypothesis that elevated α-tocopherol intakes alter gene expression of hepatic xenobiotic pathways, mice were fed diets supplemented with either 1000 IU (+E) or 35 IU (E) all-rac-α-tocopheryl acetate for 4 months; liver RNA was isolated, and gene expression was determined using both whole genome microarray and real-time quantitative polymerase chain reaction analyses. Hepatic α-tocopherol (173±18 vs. 21±1 nmol/g, mean±S.E.) and its metabolite (2,5,7,8-tetramethyl-2-(2′-carboxyethyl)-6-hydroxychroman, 0.232±0.046 vs. 0.031±0.019 nmol/g) concentrations were approximately eightfold higher following the +E dietary treatment. In +E relative to E mice, gene expression of Phase I enzymes, P450 oxidoreductase and cytochrome P450 3a11 increased 1.6-and 4.0-fold, respectively; two Phase II genes, sulfotransferase 2a and glutathione S-transferase mu 3, increased 10.8-and 1.9fold respectively, and a Phase III biliary transporter, Abcb1a, doubled. Thus, consumption of high-level dietary α-to

Who reads Alpha-tocopherol Modulates Genes Involved in Hepatic Xenobiotic Pathways in Mice?

Author

Debbie J. Mustacich; Kishorchandra Gohil; Richard S. Bruno; Michelle Yan; Scott W. Leonard; Emily Ho; Carroll E. Cross; Maret G. Traber

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0955-2863)

Published

2009

Language

EN

Field

Medicine (Health Sciences)