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Activation of Retinoic Acid Signalling After Sciatic Nerve Injury: Up-regulation of Cellular Retinoid Binding Proteins by Nina Zhelyaznik; Kirsten Schrage; Peter McCaffery; Jörg Mey is a Neuroscience article available to read on EtoBox.

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## Abstract In mammalian peripheral nerves a crush lesion causes interactions between injured neurons, Schwann cells and haematogenous macrophages that can lead to successful axonal regeneration. We suggest that the transcriptional activator retinoic acid (RA), takes part in gene regulation after peripheral nerve injury and that RA signalling is activated via the cellular retinoic acid binding protein (CRABP)‐II and cellular retinol binding protein (CRBP)‐I. With RT‐PCR and immunoblotting all necessary components of the RA signalling pathway were detected in the sciatic nerve of adult rats. These are retinoic acid receptors, retinoid X receptors, the retinoic acid synthesizing enzymes RALDH‐1, RALDH‐2, and RALDH‐3, in addition, the cellular retinoid binding proteins CRBP‐I, CRABP‐I and CRABP‐II. Enzyme activity of RALDH‐2 was detectable in the nerve, and using a transgenic reporter mouse we found local activation of RA responsive elements in the regenerating nerve. Sciatic nerve crush as well as transection resulted in a more than 10‐fold up‐regulation of CRBP‐I, which is thought to facilitate the synthesis of RA. Both kinds of injury also caused a 15‐fold increase in transcript an

Who reads Activation of Retinoic Acid Signalling After Sciatic Nerve Injury: Up-regulation of Cellular Retinoid Binding Proteins?

Author

Nina Zhelyaznik; Kirsten Schrage; Peter McCaffery; Jörg Mey

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)