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Automated synthesis of ^11^C‐β‐hydroxybutyrate by enzymatic conversion of ^11^C‐acetoacetate using β‐hydroxybutyrate dehydrogenase by Sébastien Tremblay; René Ouellet; François Bénard; Stephen C. Cunnane is a Medicine article available to read on EtoBox.

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## Abstract 1‐[^11^C]‐β‐hydroxybutyrate was produced by conversion from 1‐[^11^C]‐acetoacetate using (D)‐β‐hydroxybutyrate dehydrogenase in the presence of nicotinamide adenine dinucleotide with purification by ion exchange column chromatography. Radiochemical yield at the end of the synthesis was 10% for a total synthesis time of 36 min. High‐performance liquid chromatography analysis showed ≤4% impurities, principally unconverted acetoacetate. Residual tetrahydrofuran (34±11 ppm) and ethanol (77±30 ppm) were well under the tolerable limits for human studies. Copyright © 2008 John Wiley & Sons, Ltd.

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Author

Sébastien Tremblay; René Ouellet; François Bénard; Stephen C. Cunnane

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-2135)

Published

2008

Language

EN

Field

Medicine (Life Sciences)