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AFM and electroanalytical studies of synthetic oligonucleotide hybridization by A.-M. Chiorcea Paquim; V.C. Diculescu; T.S. Oretskaya; A.M. Oliveira Brett is a Engineering article available to read on EtoBox.

What is AFM and electroanalytical studies of synthetic oligonucleotide hybridization about?

The first and most important step in the development and manufacture of a sensitive DNA-biosensor for hybridization detection is the immobilization procedure of the nucleic acid probe on the transducer surface, maintaining its mobility and conformational flexibility. MAC Mode AFM images were used to demonstrate that oligonucleotide (ODN) molecules adsorb spontaneously at the electrode surface. After adsorption, the ODN layers were formed by molecules with restricted mobility, as well as by superposed molecules, which can lead to reduced hybridization efficiency. The images also showed the existence of pores in the adsorbed ODN film that revealed large parts of the electrode surface, and enabled non-specific adsorption of other ODNs on the uncovered areas. Electrostatic immobilization onto a clean glassy carbon electrode surface was followed by hybridization with complementary sequences and by control experiments with non-complementary sequences, studied using differential pulse voltammetry. The data obtained showed that non-specific adsorption strongly influenced the results, which depended on the sequence of the ODNs. In order to reduce the contribution of non-specific adsorbed OD

Who reads AFM and electroanalytical studies of synthetic oligonucleotide hybridization?

Author

A.-M. Chiorcea Paquim; V.C. Diculescu; T.S. Oretskaya; A.M. Oliveira Brett

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2004

Language

EN

Field

Engineering (Physical Sciences)