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Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species by Song, Ming; Li, Jingjian; Xiong, Chao; Liu, Hexia; Liang, Junsong is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species about?

Many members of the genus __Artemisia__ are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used __Artemisia__ species included __Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica__, and __Artemisia atrovirens__ were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and relia

Who reads Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species?

Author

Song, Ming; Li, Jingjian; Xiong, Chao; Liu, Hexia; Liang, Junsong

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2016

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)